Chemistry for Imaging in-situ Protein Palmitoylation

Click on Chemistry for Imaging in-situ Protein Palmitoylation throughout the Asexual Levels of Plasmodium falciparum

Palmitoylation refers back to the modification of the cysteine thiols in proteins by fatty acids, mostly palmitic acid, via ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). Palmitoylation has just lately emerged as a vital post-translational modification in malarial parasites. The expression and exercise of palmitoyl transferases differ throughout totally different developmental phases of the malarial parasite’s life cycle. The abundance of palmitoylated proteins at a given stage is a measure of total PAT exercise. The PAT exercise may also change in response to exterior alerts or inhibitors.

Right here, we describe a protocol to ‘picture’ palmitoyl-transferase exercise throughout the asexual phases utilizing Click on Chemistry and fluorescence microscopy. This methodology is predicated on metabolic labeling of a clickable analog of palmitic acid by parasitic cells, adopted by CuAAC (Copper-catalyzed Alkyne-Azide Cycloaddition response) Click on Chemistry to render palmitoylated proteins fluorescent. Fluorescence permits the quantitation of intracellular palmitoylation in parasite cells throughout numerous improvement phases. Utilizing this methodology, we noticed that intracellular palmitoylation will increase because the parasite transitions from ring to schizont phases and seems to be most plentiful throughout the schizont phases in Plasmodium falciparum.

mmune subtraction for improved decision in serum protein immunofixation electrophoresis and antibody isotype willpower in a affected person with autoantibody

Heavy chain isotypes of low degree monoclonal immunoglobulins are typically obscured in serum immunofixation electrophoresis (SIFE) by a heavy background of polyclonal immunoglobulins. Nevertheless, correct willpower of the heavy chain isotype is crucial for an entire analysis, as isotype willpower of autoantibodies could have relevance in figuring out therapeutic procedures. Immune subtraction (IS) was employed in a affected person with neuropathy and GD1a autoantibody. IS allowed identification of the cognate heavy chain associated to a lambda gentle chain restriction famous on preliminary SIFE in addition to isotype willpower of the autoantibody.

Antisera particular to particular person heavy and lightweight chains had been used for depletion of particular immunoglobulin varieties. Depletion of kappa gentle chain related immunoglobulins allowed unequivocal willpower of the isotype of lambda gentle chain-associated low degree monoclonal band to be IgG Lambda. Selective depletion of kappa, lambda, gamma and mu heavy chain immunoglobulins was employed to find out IgG Kappa isotype of the auto-antibody.

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Nuclear pore complex protein Nup153 Antibody

20-abx109340
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Nuclear pore complex protein Nup153 Antibody

abx109340-100l 100 µl
EUR 162.5

Nuclear pore complex protein Nup93 (dye) Antibody

abx346920-100l 100 µl
EUR 250

Nuclear pore complex protein Nup93 (dye) Antibody

abx346920-50l 50 µl
EUR 162.5

Nuclear pore complex protein Nup153 Antibody (HRP)

20-abx108567
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Nuclear pore complex protein Nup153 Antibody (HRP)

abx108567-100g 100 µg
EUR 362.5

Nuclear pore complex protein Nup153 Antibody (HRP)

abx108567-20g 20 µg
EUR 162.5

Nuclear pore complex protein Nup153 Antibody (HRP)

abx108567-50g 50 µg
EUR 250

Nuclear Pore Complex antibody

10R-8370 100 ul
EUR 450
Description: Mouse monoclonal Nuclear Pore Complex antibody

Nuclear pore complex protein NUP62 (NUP62) Antibody

abx349838-96tests 96 tests
EUR 250

Nuclear pore complex protein NUP62 (NUP62) Antibody

abx349839-96tests 96 tests
EUR 250

Nuclear pore complex protein Nup153 Antibody (Biotin)

20-abx105728
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  • 100 ug
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Nuclear pore complex protein Nup153 Antibody (Biotin)

abx105728-100g 100 µg
EUR 362.5

Nuclear pore complex protein Nup153 Antibody (Biotin)

abx105728-20g 20 µg
EUR 162.5

Nuclear pore complex protein Nup153 Antibody (Biotin)

abx105728-50g 50 µg
EUR 250

Nuclear pore complex protein Nup153 (NUP153) Antibody

abx402586-100tests 100 tests
EUR 400

Nuclear pore complex protein Nup153 (NUP153) Antibody

abx402586-25tests 25 tests
EUR 287.5

Nuclear pore complex protein Nup93 (dye) Antibody (HRP)

abx346921-100g 100 µg
EUR 362.5

Nuclear pore complex protein Nup93 (dye) Antibody (HRP)

abx346921-20g 20 µg
EUR 162.5

Nuclear pore complex protein Nup93 (dye) Antibody (HRP)

abx346921-50g 50 µg
EUR 250

Nuclear pore complex protein Nup93 (dye) Antibody (Biotin)

abx346923-100g 100 µg
EUR 362.5

Nuclear pore complex protein Nup93 (dye) Antibody (Biotin)

abx346923-20g 20 µg
EUR 162.5

Nuclear pore complex protein Nup93 (dye) Antibody (Biotin)

abx346923-50g 50 µg
EUR 250

Nuclear pore complex protein Nup98-Nup96 (NUP98) Antibody

abx402602-100g 100 µg
EUR 287.5

Nuclear Pore Complex Protein Nup98/Nup96 (NUP98/NUP96) Antibody

abx235932-100g 100 µg
EUR 350

Nuclear pore complex protein Nup98 (315-360)

HY-P1730 1mg
EUR 1368

Nuclear pore complex protein Nup98 315-360

TP1781-10mg 10mg Ask for price
Description: Nuclear pore complex protein Nup98 315-360

Nuclear pore complex protein Nup98 315-360

TP1781-1g 1g Ask for price
Description: Nuclear pore complex protein Nup98 315-360

Nuclear pore complex protein Nup98 315-360

TP1781-1mg 1mg Ask for price
Description: Nuclear pore complex protein Nup98 315-360

Nuclear pore complex protein Nup98 315-360

TP1781-50mg 50mg Ask for price
Description: Nuclear pore complex protein Nup98 315-360

Nuclear pore complex protein Nup98 315-360

TP1781-5mg 5mg Ask for price
Description: Nuclear pore complex protein Nup98 315-360

Nuclear Pore Complex-Interacting Protein-Like 2 (NPIPL2) Antibody

abx034938-400ul 400 ul
EUR 627.6

Nuclear Pore Complex-Interacting Protein-Like 2 (NPIPL2) Antibody

abx034938-80l 80 µl
EUR 343.2

Nuclear Pore Complex-Interacting Protein-Like 2 (NPIPL2) Antibody

abx034938-100g 100 µg
EUR 281.25

Human Nuclear pore complex protein Nup153 (NUP153)

1-CSB-EP016190HU
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Description: Recombinant Human Nuclear pore complex protein Nup153(NUP153),partial expressed in E.coli

Recombinant human Nuclear pore complex protein Nup133

P2220 100ug Ask for price
Description: Recombinant protein for human Nuclear pore complex protein Nup133

Nuclear pore membrane glycoprotein 210 Polyclonal Conjugated Antibody

C42276 100ul
EUR 476.4

Recombinant Human Nuclear pore complex protein Nup85 (NUP85)

CSB-EP874821HU 10540 mg Ask for price

Recombinant Human Nuclear pore complex protein Nup85 (NUP85)

CSB-EP874821HUl9 10977 mg Ask for price

Anti-Yeast Nuclear Pore Complex (Mouse Monoclonal) Antibody

YA39C7 100 µg
EUR 255

Mouse Nuclear pore complex protein Nup85, Nup85 ELISA KIT

ELI-23492m 96 Tests
EUR 1038

Mouse Nuclear pore complex protein Nup93, Nup93 ELISA KIT

ELI-44561m 96 Tests
EUR 1038

Human Nuclear pore complex protein Nup85, NUP85 ELISA KIT

ELI-36779h 96 Tests
EUR 988.8

Human Nuclear pore complex protein Nup88, NUP88 ELISA KIT

ELI-36780h 96 Tests
EUR 988.8

Human Nuclear pore complex protein Nup93, NUP93 ELISA KIT

ELI-36781h 96 Tests
EUR 988.8

Mouse Nuclear pore complex protein Nup50, Nup50 ELISA KIT

ELI-15055m 96 Tests
EUR 1038

Mouse Nuclear pore complex protein Nup54, Nup54 ELISA KIT

ELI-21319m 96 Tests
EUR 1038

Human Nuclear pore complex protein Nup50, NUP50 ELISA KIT

ELI-22432h 96 Tests
EUR 988.8

Mouse Nuclear pore complex protein Nup88, Nup88 ELISA KIT

ELI-35536m 96 Tests
EUR 1038

Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit

EK11485 96Т
EUR 799

Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit

AE60270RA-48Tests 48 Tests
EUR 325
Description: Rat (Rattus norvegicus)

Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit

AE60270RA-96Tests 96 Tests
EUR 610
Description: Rat (Rattus norvegicus)

Mouse Nuclear pore complex protein Nup54 (NUP54) ELISA Kit

abx536286-96tests 96 tests
EUR 687.5

Mouse Nuclear pore complex protein Nup88 (NUP88) ELISA Kit

abx536296-96tests 96 tests
EUR 687.5

Mouse Nuclear pore complex protein Nup93 (NUP93) ELISA Kit

abx536300-96tests 96 tests
EUR 687.5

Bovine Nuclear pore complex protein Nup85, NUP85 ELISA KIT

ELI-23491b 96 Tests
EUR 1113.6

Bovine Nuclear pore complex protein Nup93, NUP93 ELISA KIT

ELI-44229b 96 Tests
EUR 1113.6

Mouse Nuclear pore complex protein Nup155, Nup155 ELISA KIT

ELI-44221m 96 Tests
EUR 1038

Human Nuclear pore complex protein Nup214, NUP214 ELISA KIT

ELI-44222h 96 Tests
EUR 988.8

Mouse Nuclear pore complex protein Nup133, Nup133 ELISA KIT

ELI-44499m 96 Tests
EUR 1038

Human Nuclear pore complex protein Nup153, NUP153 ELISA KIT

ELI-44867h 96 Tests
EUR 988.8

Human Nuclear pore complex protein Nup133, NUP133 ELISA KIT

ELI-46087h 96 Tests
EUR 988.8

Human Nuclear pore complex protein Nup107, NUP107 ELISA KIT

ELI-36840h 96 Tests
EUR 988.8

Mouse Nuclear pore complex protein Nup107, Nup107 ELISA KIT

ELI-38239m 96 Tests
EUR 1038

Human Nuclear pore complex protein Nup160, NUP160 ELISA KIT

ELI-38240h 96 Tests
EUR 988.8

Human Nuclear pore complex protein Nup205, NUP205 ELISA KIT

ELI-38242h 96 Tests
EUR 988.8

Human Nuclear pore complex protein Nup155, NUP155 ELISA KIT

ELI-15040h 96 Tests
EUR 988.8

Mouse Nuclear pore complex protein Nup160, Nup160 ELISA KIT

ELI-21357m 96 Tests
EUR 1038

Mouse Nuclear pore complex protein Nup214, Nup214 ELISA KIT

ELI-21358m 96 Tests
EUR 1038

Panspecific Nuclear Pore Complex Marker

MO22107 500 ul
EUR 412.8

ELISA kit for Rat Nuclear pore complex protein Nup153 (NUP153)

KTE100879-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Rat Nuclear pore complex protein Nup153 (NUP153) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Nuclear pore complex protein Nup153 (NUP153)

KTE100879-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Rat Nuclear pore complex protein Nup153 (NUP153) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Nuclear pore complex protein Nup153 (NUP153)

KTE100879-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Rat Nuclear pore complex protein Nup153 (NUP153) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Nuclear pore complex protein Nup98- Nup96, NUP98 ELISA KIT

ELI-13775h 96 Tests
EUR 988.8

Recombinant Human Nuclear pore complex protein Nup153 (NUP153), partial

CSB-EP016190HU 1833 mg Ask for price

Recombinant Human Nuclear pore complex protein Nup153(NUP153),partial

AP70600 1mg
EUR 1978

Human Nuclear pore complex- interacting protein, NPIP ELISA KIT

ELI-16689h 96 Tests
EUR 988.8

Nuclear Pore Complex Marker (pan) mouse monoclonal antibody, clone 39C7

MO22107-500 500 µl Ask for price

NPIP (untagged)-Human nuclear pore complex interacting protein (NPIP)

SC115761 10 µg Ask for price

Recombinant Human Nuclear pore complex protein Nup98-Nup96 (NUP98), partial

CSB-MP016209HU 8119 mg Ask for price

Recombinant Human Nuclear pore complex protein Nup98-Nup96 (NUP98) ,partial

RPC27839-100ug 100ug
EUR 1158.9

Recombinant Human Nuclear pore complex protein Nup98-Nup96 (NUP98) ,partial

RPC27839-20ug 20ug
EUR 457.4

Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-10ug

QP6439-ec-10ug 10ug
EUR 240

Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-1mg

QP6439-ec-1mg 1mg
EUR 1958.4

Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-50ug

QP6439-ec-50ug 50ug
EUR 315.6

Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-100ug

QP6439-ec-100ug 100ug
EUR 489.6

Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-200ug

QP6439-ec-200ug 200ug
EUR 760.8

Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-500ug

QP6439-ec-500ug 500ug
EUR 1272

Nuclear Pore Complex Marker (pan) mouse monoclonal antibody, clone 39C7, Purified

AP20131PU-N 500 µl Ask for price

Human Nuclear pore complex-interacting protein-like 1 (NPIPB7) ELISA Kit

abx535715-96tests 96 tests
EUR 687.5

SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

20-abx123660
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SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

20-abx126518
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SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

abx237677-100ug 100 ug
EUR 610.8

SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

abx025879-400ul 400 ul
EUR 627.6

SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

abx025879-80l 80 µl
EUR 343.2

SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

abx237677-100g 100 µg
EUR 350

SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

abx126518-100l 100 µl
EUR 175

SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

abx126518-1ml 1 ml
EUR 400

SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

abx126518-200l 200 µl
EUR 275

SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody

abx025879-400l 400 µl
EUR 518.75

NPIPL3 (untagged)-Human nuclear pore complex interacting protein-like 3 (NPIPL3)

SC316960 10 µg Ask for price

Human Nuclear pore complex-interacting protein family member A1 (NPIPA1) ELISA Kit

abx535681-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member A2 (NPIPA2) ELISA Kit

abx535682-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member A3 (NPIPA3) ELISA Kit

abx535683-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member A5 (NPIPA5) ELISA Kit

abx535684-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member A7 (NPIPA7) ELISA Kit

abx535685-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member A8 (NPIPA8) ELISA Kit

abx535686-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member B3 (NPIPB3) ELISA Kit

abx535687-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member B4 (NPIPB4) ELISA Kit

abx535688-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member B5 (NPIPB5) ELISA Kit

abx535689-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member B6 (NPIPB6) ELISA Kit

abx535690-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member B8 (NPIPB8) ELISA Kit

abx535692-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member B9 (NPIPB9) ELISA Kit

abx535693-96tests 96 tests
EUR 687.5

Nuclear Matrix Protein p84 Conjugated Antibody

C49738 100ul
EUR 476.4

Human Nuclear pore complex-interacting protein family member B11 (NPIPB11) ELISA Kit

abx535646-96tests 96 tests
EUR 687.5

Human Nuclear pore complex-interacting protein family member B15 (NPIPB15) ELISA Kit

abx535647-96tests 96 tests
EUR 687.5

NPIPA3 (untagged) - Human nuclear pore complex interacting protein family, member A3 (NPIPA3)

SC335534 10 µg Ask for price

NPIPA2 (untagged) - Human nuclear pore complex interacting protein family, member A2 (NPIPA2)

SC335535 10 µg Ask for price

NPIPA5 (untagged) - Human nuclear pore complex interacting protein family, member A5 (NPIPA5)

SC335536 10 µg Ask for price

NPIPA7 (untagged) - Human nuclear pore complex interacting protein family, member A7 (NPIPA7)

SC335666 10 µg Ask for price

NPIPA8 (untagged) - Human nuclear pore complex interacting protein family, member A8 (NPIPA8)

SC335667 10 µg Ask for price

NPIPB6 (untagged) - Human nuclear pore complex interacting protein family, member B6 (NPIPB6)

SC336044 10 µg Ask for price

LOC100132247 (untagged)-Human nuclear pore complex interacting protein related gene (LOC100132247)

SC326342 10 µg Ask for price

Human Elongator Complex Protein 4 (ELP4) AssayLite™ Antibody (FITC Conjugate)

32270-05141 150 ug
EUR 370

Nuclease P1 Antibody, FITC conjugated

1-CSB-PA328459LC01PEU
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  • 100ug
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Description: A polyclonal antibody against Nuclease P1. Recognizes Nuclease P1 from Penicillium citrinum. This antibody is FITC conjugated. Tested in the following application: ELISA

NPIPA3 (myc-DDK-tagged) - Human nuclear pore complex interacting protein family, member A3 (NPIPA3)

RC237640 10 µg Ask for price

NPIPA2 (myc-DDK-tagged) - Human nuclear pore complex interacting protein family, member A2 (NPIPA2)

RC237641 10 µg Ask for price

NPIPA5 (myc-DDK-tagged) - Human nuclear pore complex interacting protein family, member A5 (NPIPA5)

RC237642 10 µg Ask for price

NPIPA7 (myc-DDK-tagged) - Human nuclear pore complex interacting protein family, member A7 (NPIPA7)

RC237772 10 µg Ask for price

NPIPA8 (myc-DDK-tagged) - Human nuclear pore complex interacting protein family, member A8 (NPIPA8)

RC237773 10 µg Ask for price

NPIPB6 (myc-DDK-tagged) - Human nuclear pore complex interacting protein family, member B6 (NPIPB6)

RC238150 10 µg Ask for price

NPIP (GFP-tagged) - Human nuclear pore complex interacting protein (NPIP). Note: ORF is codon optimized

RG219505 10 µg Ask for price

Pyruvate Dehydrogenase Complex (PDH Complex) AssayLite™ Antibody (FITC Conjugate)

30022-05141 150 ug
EUR 370

3`UTR clone of nuclear pore complex interacting protein-like 3 (NPIPL3) for miRNA target validation

SC201575 10 µg Ask for price

Anti-Nuclear pore Complex antibody

STJ120173 100 µl
EUR 562.8

Nuclease P1 Polyclonal Antibody, FITC Conjugated

A64320
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  • 100 µg
  • 50 ul
  • 100 ul

NPIP (Myc-DDK-tagged)-Human nuclear pore complex interacting protein (NPIP). Note: ORF is codon optimized

RC219505 10 µg Ask for price

Nuclear pore glycoprotein p62 (NUP62) Antibody (FITC)

abx343986-100l 100 µl
EUR 250

Nuclear pore glycoprotein p62 (NUP62) Antibody (FITC)

abx343986-50l 50 µl
EUR 162.5

Nuclear pore membrane glycoprotein 210 Antibody (FITC)

20-abx107147
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NPIPB9 (untagged) - Human nuclear pore complex interacting protein family, member B9 (NPIPB9), transcript variant 2

SC336069 10 µg Ask for price

NPIPB9 (untagged) - Human nuclear pore complex interacting protein family, member B9 (NPIPB9), transcript variant 1

SC336087 10 µg Ask for price

Human Nuclear Cap-Binding Protein Subunit 2 (NCBP2) AssayLite™ Antibody (FITC Conjugate)

30189-05141 150 ug
EUR 370

NPIPL3 (GFP-tagged) - Human nuclear pore complex interacting protein-like 3 (NPIPL3). Note: ORF is codon optimized

RG216779 10 µg Ask for price

Pyruvate Dehydrogenase Complex (PDH Complex) AssayLite Antibody (FITC, RPE, APC, PerCP Conjugate)

30022-05181 1 x 75 ug, 3 x 30 ug
EUR 702

NPIPL3 (Myc-DDK-tagged)-Human nuclear pore complex interacting protein-like 3 (NPIPL3). Note: ORF is codon optimized

RC216779 10 µg Ask for price

NPIPB9 (myc-DDK-tagged) - Human nuclear pore complex interacting protein family, member B9 (NPIPB9), transcript variant 2

RC238175 10 µg Ask for price

NPIPB9 (myc-DDK-tagged) - Human nuclear pore complex interacting protein family, member B9 (NPIPB9), transcript variant 1

RC238193 10 µg Ask for price

Actin-related protein 2/3 complex subunit 3 Polyclonal Conjugated Antibody

C42389 100ul
EUR 476.4

Rat SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) ELISA Kit

abx545234-96tests 96 tests
EUR 687.5

Histone Complex (H1, H2A, H2B, H3, H4) AssayLite™ Antibody (FITC Conjugate)

30200-05141 150 ug
EUR 370

Proteasome activator complex subunit 3 Polyclonal Conjugated Antibody

C42571 100ul
EUR 476.4

Human SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) ELISA Kit

abx383083-96tests 96 tests
EUR 1093.2

Nuclear Receptor NR4A1 (Ab-351) Conjugated Antibody

C33181 100ul
EUR 476.4

Histone Core Complex (H2A, H2B, H3, H4) AssayLite™ Antibody (FITC Conjugate)

30198-05141 150 ug
EUR 370

LOC100132247 (GFP-tagged) - Human nuclear pore complex interacting protein related gene (LOC100132247). Note: ORF is codon optimized

RG227697 10 µg Ask for price

Nucleoprotein Polyclonal Antibody, FITC Conjugated

A55509
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  • 100 µg
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  • 100 ul

Nucleoprotein Polyclonal Antibody, FITC Conjugated

A55012 100 µg
EUR 684.66

Antifreeze Protein Supplementation In the course of the Warming of Vitrified Bovine Ovarian Tissue Can Enhance the Ovarian Tissue High quality After Xenotransplantation

 

The prevalence of ice crystallization throughout ovarian tissue (OT) cryopreservation causes unavoidable cryodamage, and ice recrystallization throughout the warming is extra detrimental than ice crystallization. Right here, we investigated that antifreeze protein (AFP) therapy throughout the warming process can enhance the bovine OT high quality after xenotransplantation (XT). Bovine OTs (n=120) had been evenly assigned to 4 teams: contemporary, vitrified-warmed, vitrified-warmed with 10 mg/mL Leucosporidium ice-binding protein (LeIBP, a sort of AFP) (LeIBP-10), and vitrified-warmed with 20 mg/mL LeIBP (LeiBP-20). LeIBPs had been added to the primary warming answer. Twenty items of OTs had been assigned to every class. The remaining 10 OTs from every class had been assigned to the XT-Recent management, XT-Vitrified-warmed management, XT-LeIBP-10, and XT-LeIBP-20 teams, respectively, and xenotransplanted to 9-week-old ovariectomized nude mice for one week.

LeIBP therapy throughout the warming step elevated morphological follicle normality and decreased apoptotic follicle ratios after vitrification-warming and XT. The XT-vitrified-warmed management group confirmed considerably decreased microvessel density and elevated fibrosis when in comparison with that of the XT-fresh group. Microvessel density and fibrosis had been recovered in each LeIBP handled teams. There was no vital distinction between the LeIBP-10 and LeIBP-20 teams in all outcomes. AFP therapy throughout the warming process can stop OT injury, and enhance ovarian follicle morphology and apoptosis in each the vitrified-warmed bovine OT and its graft. After affirmation in a human examine, AFPs can doubtlessly be utilized to human OT cryopreservation to cut back cryodamage and enhance the OT high quality.

Attenuated Induction of the Unfolded Protein Response in Grownup Human Major Astrocytes in Response to Recurrent Low Glucose

Goals/speculation: Recurrent hypoglycaemia (RH) is a serious side-effect of intensive insulin remedy for folks with diabetes. Modifications in hypoglycaemia sensing by the mind contribute to the event of impaired counterregulatory responses to and consciousness of hypoglycaemia. Little is understood in regards to the intrinsic adjustments in human astrocytes in response to acute and recurrent low glucose (RLG) publicity.

Strategies: Human main astrocytes (HPA) had been uncovered to zero, one, three or 4 bouts of low glucose (0.1 mmol/l) for 3 hours per day for 4 days to imitate RH. On the fourth day, DNA and RNA had been collected. Differential gene expression and ontology analyses had been carried out utilizing DESeq2 and GOseq, respectively. DNA methylation was assessed utilizing the Infinium MethylationEPIC BeadChip platform.

Outcomes: 24 differentially expressed genes (DEGs) had been detected (after correction for a number of comparisons). One bout of low glucose publicity had the most important impact on gene expression. Pathway analyses revealed that endoplasmic-reticulum (ER) stress-related genes comparable to HSPA5XBP1, and MANF, concerned within the unfolded protein response (UPR), had been all considerably elevated following low glucose (LG) publicity, which was diminished following RLG. There was little correlation between differentially methylated positions and adjustments in gene expression but the variety of bouts of LG publicity produced distinct methylation signatures.

Conclusions/interpretation: These knowledge counsel that publicity of human astrocytes to transient LG triggers activation of genes concerned within the UPR linked to endoplasmic reticulum (ER) stress. Following RLG, the activation of UPR associated genes was diminished, suggesting attenuated ER stress. This can be a consequence of a profitable metabolic adaptation, as beforehand reported, that higher preserves intracellular vitality ranges and a decreased necessity for the UPR.

Doxorubicin/Nucleophosmin Binding Protein-Conjugated Nanoparticle Enhances Anti-leukemia Exercise in Acute Lymphoblastic Leukemia Cells in vitro and in vivo

Acute lymphoblastic leukemia (ALL) is an aggressive malignancy. Adults with ALL have greater than 50% relapse charges. We have now beforehand validated that overexpression of nucleophosmin (NPM) is concerned within the multidrug resistance (MDR) improvement throughout ALL; and a synthetically engineered recombinant NPM binding protein (NPMBP) has been developed in our group; NPMBP and doxorubicin (DOX) could be conjugated in a nanoparticle-based drug supply system named DOX-PMs-NPMBP to counteract MDR throughout ALL. Right here, we evaluated the antileukemia potential of DOX-PMs-NPMBP in resistant ALL cells. This examine demonstrates that DOX-PMs-NPMBP considerably enhances chemosensitivity to DOX in ALL cells.

Regardless of at variable concentrations, each resistant and first ALL cells from relapsed sufferers had been delicate to DOX-PMs-NPMBP. Intimately, the half maximal inhibitory focus (IC50) values of DOX-PMs-NPMBP had been between 1.6- and seven.0-fold decrease than these of DOX in cell strains and first ALL cells, respectively; and apoptotic cells ratio was over 2-fold increased in DOX-PMs-NPMBP than DOX. Mechanistically, p53-driven apoptosis induction and cell cycle arrest performed important position in DOX-PMs-NPMBP-induced anti-leukemia results. Furthermore, DOX-PMs-NPMBP considerably inhibited tumor progress and extended mouse survival of ALL xenograft fashions; and no systemic toxicity prevalence was noticed after therapy throughout follow-up. In conclusion, these knowledge point out that DOX-PMs-NPMBP could considerably exert progress inhibition and apoptosis induction, and markedly enhance DOX antileukemia exercise in resistant ALL cells. This novel drug supply system could also be invaluable to develop as a brand new therapeutic technique towards multidrug resistant ALL.

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